- Prepare media
a. PDA- Potato Dextrose Agar Medium: Potato (250g), dextrose (20g), agar (20g), water (1000ml)
b. MEA – Malt Extract Agar Medium: malt extract (30g), agar (15 g), water (1000 ml);
c. YMA – Yeast Malt Agar Medium: malt (20g), yeast (2g), agar (15g), water (1000 ml).
2. Mycelium inoculation
The treatment medium was inoculated with a 14-day-old agar mycelial plug (5mm) of oyster mushroom. under aseptic conditions. Inoculum was placed in the middle of each petri dish and special care was taken to keep the cylinder mycelium in direct contact with each substrate. Radial growth of mycelium of different portions was observed until the Petri dishes were filled with it. The plates were incubated at 240C and observed for 7 days during which the mycelial vegetative growth and mycelial density of Pleurotus sp. were recorded. The growth rate is given by the formula below: Growth rate=Colony diameter on the last day (cm) /Number of day’s measurement was taken after inoculation. Daily mycelial growth was determined using a ruler across the Petri-dish horizontally.