Medium for Cordyceps militaris Pure Culture
Maltose Yeast Extract Peptone Sucrose Medium (MYPS)
- 4 g maltose,
- 6 g peptone,
- 4 g yeast extract
- 10 g sucrose
- 20 g agar
- 1000 mL distilled water
Crapek -Dox (C-Dox) Media
- – 30g sucrose
- – 2g NaNO3
- – 1g KH2PO4
- – 0.5g MgSO4.7H2O
- – 0.5g KCl
- – 20g agar powder
- – 1000mL distilled water
Potato Dextrose Agar (PDA)
- 200 g potatoes
- 20 g dextrose
- 30 g sucrose
- 20 g agar powder
- 1000 mL distilled water.
All of the media were adjusted to pH 7 with 1 N NaOH or HCl.
The mycelium discs of each C. militaris were placed in petri dishes containing each culture medium (20 mL) and incubated at 24 0C in darkness.
