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Medium for Cordyceps militaris Pure Culture

Cordyceps militaris Pure Culture

Maltose Yeast Extract Peptone Sucrose Medium (MYPS)

  • 4 g maltose,
  • 6 g peptone,
  • 4 g yeast extract
  • 10 g sucrose
  • 20 g agar
  • 1000 mL distilled water

Crapek -Dox (C-Dox) Media

  • – 30g sucrose
  • – 2g NaNO3
  • – 1g KH2PO4
  • – 0.5g MgSO4.7H2O
  • – 0.5g KCl
  • – 20g agar powder
  • – 1000mL distilled water

Potato Dextrose Agar (PDA)

  • 200 g potatoes
  • 20 g dextrose
  • 30 g sucrose
  • 20 g agar powder
  • 1000 mL distilled water.
Cordyceps militaris Pure Culture

All of the media were adjusted to pH 7 with 1 N NaOH or HCl.

The mycelium discs of each C. militaris were placed in petri dishes containing each culture medium (20 mL) and incubated at 24 0C in darkness.

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